Nanomaterial-based electrochemical sensing platform for the determination of Olaparib

Abstract

Olaparib is an anticancer drug used as a maintenance treatment for certain cancers such as pancreatic, prostate, ovarian, fallopian tube, peritoneal, and breast cancer. Due to its great importance for cancer treatment, we aimed to develop and validate an electrochemical method for the determination and the quantitation of Olaparib. Electrochemical methods are inexpensive, user-friendly, highly sensitive, and have short measurement times compared to conventional analysis methods. Besides, the sensitivity of the sensors is enhanced by modifying the surface of the electrodes by using different materials. In this study, the GCE-modified multi-walled carbon nanotubes (MWCNT) and iron (III) oxide (Fe2O3) nanoparticles were applied for the determination of Olaparib by differential pulse voltammetry (DPV). The irreversible electron transfer processes between Olaparib and the electrode surface was indicated the behavior of peak. The linear concentrations of Olaparib were measured from 0.4 to 40 µM in 0.5 M H2SO4. The limit of determination (LOD) and limit of quantification (LOQ) were 6.4 nM and 21 nM, respectively. The nanomaterial-modified electrode was successfully used for the analysis of Olaparib in pharmaceutical dosage form and commercial serum samples. In addition, a plausible oxidation mechanism of Olaparib was proposed yielding to its potential oxidation metabolite Olaparib-OX. Furthermore, molecular, electronic properties and irreversible electrochemical oxidation process of Olaparib were discussed using the density functional theory (DFT).