Nanomagnetic wheat gluten hydrolysates a new carrier for nanoimmobilization of inulinase

Abstract

Functionalized magnetic nanoparticles are effective enzyme carriers since they have several advantages such as easily separation under external magnetic fields as well as reusability of enzymes. In this study, it was tried to prepare a composite nanoparticle with Fe3O4 nanoparticles and wheat gluten hydrolysate nanoparticles for utilizing in biocatalyst production. Firstly, Fe3O4 nanoparticles were synthesized by chemical co-precipitation. Since these nanoparticles are easily aggregated, surface coating procedure was used. These nanoparticles were coated by wheat gluten hydrolysates (WGHs) using glutaraldehyde as crosslinking agent. WGHs were prepared by enzymatic hydrolysis of wheat gluten by endopeptidases with degree of hydrolysis (24.3%) and then, by desolvation method using ethanol as a desolvating agent. FE-SEM images and DLS analysis showed that Fe3O4 nanoparticles have spherical morphology with 30–40 nm in diameter. Also, wheat gluten hydrolysates were ranged 100–150 nm and their zeta potential was around −29 mV. It should be noted that the size of nanocomposite particles were about 120–160 nm. Inulinase was covalently and successfully immobilized through gluteraldehyde on Fe3O4@WGH nanoparticles. Moreover, The results implied that, the enzyme was loaded up to 75% onto functionalized nanoparticles.