Abstract
Synchrotron nanoprobe X-ray absorption (XAS) studies of a potent organo-osmium arene anticancer complex in ovarian cancer cells at subcellular resolution allow detection and quantification of both OsII and OsIII species, which are distributed heterogeneously in different areas of the cells.
Highlights
Synchrotron nanoprobe X-ray absorption (XAS) studies of a potent organo-osmium arene anticancer complex in ovarian cancer cells at subcellular resolution allow detection and quantification of both OsII and OsIII species, which are distributed heterogeneously in different areas of the cells
Synchrotron radiation is suitable for exploring the chemical behaviour of metals in biological samples.[9]
XAS is a powerful technique that can be used to obtain valuable information about the chemical state, and the electronic and structural properties of metals. This is achieved by using an X-ray beam of variable energy to probe the binding energy of electrons in specific electronic shells of the element (X-ray Absorption Near Edge Structure; XANES), or the elastic scattering processes between the photo-electrons generated by the incident beam and other atoms in the vicinity of the metal centre (Extended X-Ray Absorption Fine Structure, EXAFS) (Fig. S1, Electronic supplementary information (ESI)†)
Summary
Synchrotron nanoprobe X-ray absorption (XAS) studies of a potent organo-osmium arene anticancer complex in ovarian cancer cells at subcellular resolution allow detection and quantification of both OsII and OsIII species, which are distributed heterogeneously in different areas of the cells. The XAS spectra were recorded for a series of OsII standards that might represent species that could be found inside cells, based on the in vitro activation experiments (1, 1-OH, 1-Cl and 1-SG; Scheme S1 and Fig. S6, ESI†),[7] and Os0 to OsIV standards to probe the oxidation state of intracellular Os species.
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