Abstract
Nanofluidic chips with different numbers of nanochannels were fabricated based on a commercial AFM system using a single-scratch approach. The electrical characterization and enzymatic reactions at the nanoscale were demonstrated using the obtained chips. The effects of the number of nanochannels and the solution concentration on the measured electric current were investigated. The influence of the hydrodynamic convection generated from the induced inflow at the end of the nanochannel on the ion transport through the nanochannel was also studied. Moreover, the enzymatic reactions for trypsin towards poly-l-lysine (PLL) or thrombin were conducted with a nanofluidic chip to investigate the reaction specificity between trypsin and PLL. Results show that the electric current change during the experimental process could be used as a label-free indicator to detect the enzymatic activity.
Highlights
Nano uidic devices have drawn signi cant attention because of their potential application in various elds, such as health care,[1] medicine,[2] and DNA/protein transport.[3,4] Fabrication of nano uidic chips using a simple and highly efficient approach has become a research focus and challenge for the application of nano uidic devices
It is inconvenient for photolithography approach to change the photomasks when fabricate different micro/nanostructures
In this study, nano uidic chips with single or multiple nanochannels were prepared based on a commercial atomic force microscopy (AFM) system using the single scratching approach
Summary
Nano uidic devices have drawn signi cant attention because of their potential application in various elds, such as health care,[1] medicine,[2] and DNA/protein transport.[3,4] Fabrication of nano uidic chips using a simple and highly efficient approach has become a research focus and challenge for the application of nano uidic devices. The nanochannels of the nano uidic chips prepared in this work were fabricated using a single scratch approach. Nano uidic chips can be used to study ion transport characterization in nanochannels.
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