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Abstract
Model test-systems for biochemical determination of glucose and cholesterol are designed by covalent coupling of enzymes to the surface of modified nanodiamonds (MND). In both cases after immobilization on MND the enzymes exhibit activity and catalyze the formation of a colored product in the chain of successive enzymatic reactions of oxidative azocoupling (Н2О2—4-aminoantipyrine—phenol). Produced MND-enzymes complexes: function in DI water and various buffers, over a wide range of temperature and pH, provide for the linear product yield over a broad concentration range of analyzed substances, make possible to test cholesterol and glucose in vitro many times, exhibit functional activity after long storage at + 4°C.
Highlights
IntroductionDetonation nanodiamonds generate much interest in researchers, because physical-chemical properties of these nanoparticles (small size, reactive surface, low toxicity, high biocompatibility, etc.) make possible to forecast a broad range of their biomedical applications [1,2,3,4]
Detonation nanodiamonds generate much interest in researchers, because physical-chemical properties of these nanoparticles make possible to forecast a broad range of their biomedical applications [1,2,3,4]
We investigated the possibility of conjugating modified nanodiamonds (MND) with enzymes to the design of the biochemical diagnostic test-systems for evaluation of cholesterol and glucose
Summary
Detonation nanodiamonds generate much interest in researchers, because physical-chemical properties of these nanoparticles (small size, reactive surface, low toxicity, high biocompatibility, etc.) make possible to forecast a broad range of their biomedical applications [1,2,3,4]. Among these applications is new biochemical indicator means (including reusable systems) to broaden the inventory of medical diagnostic aids. For experts in the field of interest can be modified nanodiamonds (MND) which form free-dispersed systems and adapted for medical-biological study [5,6]. Polymorphous active surface of MND featuring high colloidal stability in aqueous media [9,10] suggests its use to develop tools for biochemical diagnosis of components of biological liquids.
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