Abstract
In response to the demand for new multifunctional materials characterized by high biocompatibility, hydrogel (HG) nanocomposites as a platform for bioactive compound delivery have been developed and fabricated. A specific crosslinking/copolymerization chemistry was used to construct hydrogels with a controlled network organization. The hydrogels were prepared using 3,6-anhydro-α-l-galacto-β-d-galactan (galactose hydrogel) together with resveratrol (trans-3,5,4′-trihydroxystilbene) and calcium hydroxyapatite nanoparticles. The resveratrol was introduced in three different concentrations of 0.1, 0.5, and 1 mM. Nanosized calcium hydroxyapatite was synthesized by a microwave-assisted hydrothermal technique, annealed at 500 °C for 3 h, and introduced at a concentration 10% (m/v). The morphology and structural properties of Ca10(PO4)6(OH)2 and its composite were determined by using XRPD (X-ray powder diffraction) techniques, as well as the absorption and IR (infrared) spectroscopy. The average nanoparticle size was 35 nm. The water affinity, morphology, organic compound release profile, and cytocompatibility of the obtained materials were studied in detail. The designed hydrogels were shown to be materials of biological relevance and of great pharmacological potential as carriers for bioactive compound delivery. Their cytocompatibility was tested using a model of human multipotent stromal cells isolated from adipose tissue (hASCs). The biomaterials increased the proliferative activity and viability of hASCs, as well as reduced markers of oxidative stress. In light of the obtained results, it has been thought that the designed materials meet the requirements of the tissue engineering triad, and may find application in regenerative medicine, especially for personalized therapies.
Highlights
Multifunctional hybrid materials, i.e., those that are organic/inorganic, are able to respond, reversibly, to more than one external stimulus
The Influence of Hydrogels Functionalized with nanocrystalline hydroxyapatite (nHAp) and Resveratrol on human adipose-derived mesenchymal stem cells (hASCs) Morphology, Proliferation, and Metabolic Activity
The analysis revealed that functionalization of hydrogels with nHAp and resveratrol positively affected the growth pattern and metabolic activity of hASCs
Summary
Multifunctional hybrid materials, i.e., those that are organic/inorganic, are able to respond, reversibly, to more than one external stimulus. A new strategy has been proposed for the deposition of calcium hydroxyapatite into biodegradable hydrogels This modification has led to the mechanical improvement of the material, preserving its biocompatibility and providing possible controlled release at the target site of action. The cytocompatibility of obtained materials was evaluated in vitro using human ASCs. it has been shown that particular doses of RES can promote the viability of the ASCs, proliferative and metabolic activity, as well as mitochondrial potential, reducing oxidative stress. It has been shown that particular doses of RES can promote the viability of the ASCs, proliferative and metabolic activity, as well as mitochondrial potential, reducing oxidative stress In this way, nHAp modified with RES could be become a future promising material for regenerative medicine
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