Nanocomposites with ornidazole\u2014antibacterial and antiadhesive agents against Gram-positive and Gram-negative bacteria

I Furtat,A Gaidai,M Lupatsii,O Biliayeva,A Golub,T Murlanova,H Sobczuk,P Vakuliuk

doi:10.1007/s13204-020-01260-x

Abstract

Antimicrobial resistance of many microbial species can cause to thousands of deaths worldwide, in this regard new therapeutic strategies have to be invented. To address the question, we have prepared nanocomposites on the basis of pyrogenic silicon dioxide with ornidazole immobilized on the surface (ornidasil) and studied their antimicrobial properties and the therapeutic potential. It has also been shown, that in comparison with pure ornidazole the addition of ornidazole to nanocomposite composition can enhance the antimicrobial spectrum, including Gram-positive and Gram-negative bacteria. The most significant bactericidal effect has been reached after more than 24-h treatment with the nanocomposite. Antiadhesive properties of nanocomposite materials were studied using blood types OO+, AO+, BO+, AB+, the degree of bacterial adhesion was estimated using three indexes: average adhesion index, index of erythrocytes involvement, index of microbial adhesion. The effectiveness of the treatment with the nanocomposites obtained was studied on complicated wounds of various etiologies, in particular the wounds caused by diabetic foot syndrome.

Highlights

Nowdays, the emergence of strains of microorganisms resistant to antimicrobial compounds is one of the leading problems, because introduction of antibiotic compounds to clinical practice has rapidly increased the resistance of the majority of clinically significant strains of microorganisms to the above mentioned compounds (Poole 2002)
Ornidazole relates to the third generation of nitroimidazoles, and it is active in the treatment of anaerobic bacteria and protozoa
The research shows that nanocomposites with ornidazole are characterized as antimicrobial and antiadhesive agents against Gram-positive and Gram-negative bacteria, which proves that this type of nanocomposites can be prospective to inhibit the spread and development of infectious diseases

Summary

Introduction

The emergence of strains of microorganisms resistant to antimicrobial compounds is one of the leading problems, because introduction of antibiotic compounds to clinical practice has rapidly increased the resistance of the majority of clinically significant strains of microorganisms to the above mentioned compounds (Poole 2002). Such a rapid increase is caused by several factors, the main ones are the overuse of antibiotics in clinical practice, inadequate use of antimicrobial compounds or their overdosage, and the intensive use of antibiotics in agriculture and industry (Ventola 2015). One of the main strategies in the development of adhesion inhibitors is the introduction of such compounds that can

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