Abstract
ABSTRACT Novel nanocomposite smart hydrogel (NSH) and smart hydrogel (SH) are prepared to be used as a support for invertase. Spectrophotometric and thermal analysis methods were used for the characterization. Lower critical solution temperature values of SH and NSH were found as 32.68°C and 30.44°C, respectively. From the pH-responsive swellings performed at three different temperatures, the inflection point values of NSH were found at higher pHs compared to SH. Invertase was immobilized onto SH and NSH (SH−I and NSH−I). The optimum pH and temperature values were found. Michaelis constant and maximum reaction rate were calculated. NSH−I showed excellent thermal, operational and pH stability.
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