Abstract
ObjectiveChitosan nanoparticle (nanochitosan) has a broad antimicrobial spectrum against diverse pathogenic microorganisms. However, its effect on dental caries-associated microorganisms, such as Streptococcus mutans and Candida albicans is yet to be explored. These microorganisms are known for causing early childhood caries. Therefore, this study was aimed at investigating nanochitosan inhibition capacity against dual-species biofilms of S. mutans and C. albicans. In this study, nanochitosan antimicrobial activity is reported against mono and dual biofilm species of S. mutans and/or C. albicans at 3 and 18 h incubation time. Nanochitosan inhibition capacity was observed through biofilm mass quantity and cell viability.ResultsThe present study successfully synthesized nanochitosan with average diameter of approximately 20–30 nm, and also established dual-species biofilms of S. mutans and C. albicans in vitro. With nanochitosan treatment, the cell viability of both microorganisms significantly decreased with the increasing concentration of nanochitosan. There was no significant decrease in biofilm mass both in the dual and single-species biofilms after 3 h of incubation. However, greater inhibition of biofilm was observed at 18 h incubation.
Highlights
Childhood caries (ECC) is an aggressive form of dental caries which affects most children (< 72 months age) in developing countries
Streptococcus mutans and Candida albicans co‐colonization The co-colonization between S. mutans and C. albicans was evaluated from biofilm mass percentage in control group
The results of present study reflect the role of nanochitosan in inhibiting C. albicans and S. mutans as
Summary
At 18-h incubation, the dual-species biofilms mass was significantly higher than the single-species biofilm mass of C. albicans and S. mutans. Evaluation of nanochitosan antimicrobial activity on dual‐species biofilms The effect of nanochitosan on dual-species biofilms was investigated through cell viability and biofilm mass changes. The cell viability of both species significantly decreased at 3-h and 18-h incubation, along with the increasing nanochitosan concentration. At 18-h incubation, it started to show a decreasing trend of remaining biofilm mass, along with the increasing nanochitosan concentration. An increase in percentage of biofilm mass reduction started to be seen with the increase in concentration of nanochitosan used at 18-h incubation
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
