Abstract
This study evaluates the capability of Komagataeibacter hansenii ATCC 23769 to synthesize bacterial nanocellulose (BNC) under strict limitation of nutrients with different carbon and nitrogen sources in a defined minimal culture medium in static culture. Five carbon sources were prepared based on the C-molar basis concentration: glycerol, glucose, fructose, mannitol and saccharose, combined with three different nitrogen sources: NH4Cl, NH4NO3 and (NH4)2SO4. BNC production yields were determined based on the dry weight of the produced BNC-Minimal membranes and the carbon consumption for each condition. The combination of 25 mM of glucose and 10 mM of NH4Cl showed the best concentration of C and N sources regarding BNC yield and membrane stability. This is a new proposal of defined minimal culture medium that supports growth of BNC membranes production, without the addition of complex elements such as yeast extract and peptone. BNC membranes produced under these conditions exhibited great optical transparency compared to the membranes produced in complex media (Mannitol and Hestrin–Schramm), therefore increasing a wide range of technological applications.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
