Abstract
The effect of nanobubbles on anaerobic growth and metabolism of Pseudomonas aeruginosa was investigated. P. aeruginosa grew earlier in the culture medium containing nanobubbles and the bacterial cell concentration in that culture medium was increased a few times higher compared to the medium without nanobubbles under anaerobic condition. Both gas and protein, which are the metabolites of P. aeruginosa, were remarkably produced in the culture medium containing nanobubbles whereas those metabolites were little detected in the medium without nanobubbles, indicating nanobubbles activated anaerobic growth and metabolism of P. aeruginosa. The carbon dioxide nanobubbles came to be positively charged by adsorbing cations and delivered ferrous ions, one of the trace essential elements for bacterial growth, to the microbial cells, which activated the growth and metabolism of P. aeruginosa. The oxygen nanobubbles activated the activities of P. aeruginosa as an oxygen source.
Highlights
The effect of nanobubbles on anaerobic growth and metabolism of Pseudomonas aeruginosa was investigated
Pseudomonas aeruginosa is widely distributed in the natural environment, and they can grow under aerobic conditions by respiration with oxygen, while they can grow under anaerobic conditions by denitrification[1,2]
The results showed that the growth and metabolic activities of P. aeruginosa were activated by the presence of nanobubbles in the culture medium
Summary
The effect of nanobubbles on anaerobic growth and metabolism of Pseudomonas aeruginosa was investigated. Bioproduction of rhamnolipid and microbial hydrocarbon degradation, which are useful for bioremediation and enhanced oil recovery, can be more proceeded under aerobic conditions[17,18,19], so that the growth and metabolic activity of P. aeruginosa must be activated even under anaerobic conditions to increase the efficacy of the objective in these microbial utilization processes. For this purpose, chemical nutrients that activate the growth and metabolism of the microorganism may be applied to the target field. Because nanobubbles can be generated from gas, both the environmental impact and cost are smaller than the chemical nutrients
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