NanoBRET – ein neues Nachweisverfahren für die GPCR-Ligandenbindung

Abstract

A number of assay techniques are currently used to study ligand binding to G protein-coupled receptors (GPCRs) in living cells. In contrast to FRET, BRET does only need the addition of a suitable substrate to generate light/energy which then excites the fluorescent binding partner. This article describes the newly developed NanoBRETTM method for monitoring ligand binding to GPCRs in live cells using a microplate reader. Saturation and competition binding experiments are shown.