Abstract
The present research was aiming to study In-Silico the effect of Glycyrrhizic Acid ammonium salt (GAS) and Salicylic acid (SA) on the coat protein of potato leafroll virus (PLRV). In addition, in-vitro studying the effect of (GAS NPs) and Salicylic acid (SA NPs) nanoparticles at concentrations 0.15, 0.30, 1.25 and 2.5 mM, respectively, to control, decline or reduce the presence of PLRV in potato plants Solanum tuberosum L. selena. (GAS NPs) and (SA NPs) were applied in the MS medium at concentrations 0.15, 0.30, 1.25 and 2.5 mM, respectively. Results revealed that, enhancement or decline the PLRV according to the initiation of specific pathways. The expression level of Kinase 3 gene increased significantly due to the two used concentrations of GAS NPs. While the expression of callose gene was upregulated significantly in response to treatment of PLRV infected plant with (GAS NPs) with concentration (0.30 mM). Treatment with (SA NPs) caused upregulation significance only of callose gene at (2.5 mM) concentration. The molecular modeling results of used compounds (glycyrrhizic acid ammonium salt and salicylic acid) showed highest score of binding and the best rms define value with a very good binding mode and perfect interactions with amino acids of the three subunits (A, B and C) forming the protein coat of leaf roll virus. Glycyrrhizic acid ammonium salt and salicylic acid nanoparticles could be perfect solution to produce potato plant free virus in-vitro. Further larger studies are needed to investigate the role of the studied compounds in vivo.
Highlights
Our research study confirmed that molecular docking can guide researchers to choose the effective material against different plant pathogen, speed up in-vitro and in-vivo application
Docking studies were conducted using the MOE 2019 drug design program to evaluate the activity of the two tested compounds against the Potato Leaf Roll viral protein coat, which has been linked to many viral characteristics such as serological properties, transmission specificity [29], cross-protection and mutual exclusion [30]
Potato leaf roll virus is composed of a pyramidal shaped protein coat divided into three subunits of nearly the same size (A, B and C) with the largest active site which is the central core located in between the three subunits
Summary
Potato crop is recognized as one of the most valuable crops worldwide. Potato crop production has increased remarkably during the last few decades in comparison with major staple crops. Potato plants could be infected with many types of viruses. Despite the fact that over 50 distinct viruses and one viroid have been reported as potato viral infections across the world, only a few of them cause significant global losses
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
