Abstract
Proliferating cell nuclear antigen (PCNA) from Sulfolobus solfataricus is a heterotrimeric ring-shaped protein. Bacterial cytochrome P450 (P450) that is one of promising biocatalysts requires electron transfer via two kinds of electron transfer proteins. Here we report that the fusion proteins of three subunits of PCNA and a bacterial P450 and its electron transfer proteins formed a quite stable heterotrimeric complex, and showed extremely high catalytic activities compared to the equimolar mixture of those. This complex formation utilizing PCNA could be valuable for nanoarchitecture of complicated multienzymatic reactions as well as applications of electron transport proteins.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
