Nano-ZnO-assisted matrix solid phase dispersion microextraction combined with an in situ DES-based aqueous two-phase system for the enrichment of multiple active compounds in honeysuckles

Abstract

In this work, a novel method of matrix solid phase dispersion-assisted aqueous two-phase system microextraction as sample pretreatment was developed, and ultra-high performance liquid chromatography was used to separate multiple active compounds in honeysuckles. Nano-ZnO was used as the adsorbent for matrix solid phase dispersion microextraction, and green deep eutectic solvents were used as the extraction solvent for enrichment of target analytes in an aqueous two-phase system. In this work, single-factor experiments and Box-Behnken experimental design combined with response surface methodology were used to study and optimize the parameters of matrix solid phase dispersion-assisted aqueous two-phase system microextraction. The fitted models extracted from chlorogenic acid, loganin, sweroside, rutin, luteoloside, isochlorogenic acid A, and isochlorogenic acid C were successfully established and validated. Based on the optimal conditions, the correlation coefficient ranges from 0.9983 to 0.9998, and the detection limits of each analyte are in the range of 1.360–13.531 ng/mL, indicating that the method has a good linear calibration curve and good sensitivity. The recoveries of the seven target analytes in honeysuckles at two levels of intensification are between 71.35% and 104.77%, and the relative standard deviations are lower than 4.28%, showing that the mentioned method has satisfactory recovery efficiency and high reproducibility. In conclusion, this study demonstrates that matrix solid phase dispersion-assisted aqueous two-phase system microextraction is a novel method that is efficient, environmentally friendly and economical and has the potential to extract multiple active components from honeysuckles.