Abstract
AbstractThe electrocatalytic oxidation of nicotinamide adenine dinucleotide (NADH) was studied on nanoTiO2 modified sol‐gel electrode, using cyclic voltammetry, chronoamperometry and differential pulse voltammetry as diagnostic techniques. It is demonstrated that TiO2 nanoparticles on sol‐gel network catalyze the oxidation of NADH in the absence of any electron transfer mediators. Effect of various parameters such as pH, scan rate, TiO2 percentage on the response of modified electrode was studied. In addition, scanning electron microscopy (SEM) was used to characterize the surface morphology of the spots. A dynamic range between 0.5–50 μM with detection limit of 0.35 μM was obtained with DPV studies. This method was successfully used for determination of NADH in cucumber cotyledons samples. The electrode showed relatively good stability over more than 2 months.
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