Nano Monitors for Identification of Vulnerable Cardio-Vascular Plaque

Abstract

AbstractAs our nation's population ages, there will be a substantial demand for surgical services. The best predictor of postoperative outcome is the presence of perioperative ischemia, which is caused by vulnerable coronary plaque rupture. It is not know what makes plaques rupture, but inflammation has been proposed as a unifying etiology. The physiologic perioperative state is one of intense, acute inflammation and thrombosis. This is characterised by the presence of protien- Human Serum C-Reactive Protein( hsCRP) and Myeloperoxidase(MPO). There is a gap in detection capability between gold standard in proteomics detection –Enzyme Linked Immunosorbent Assay (ELISA) assay methods and electrical biosensors.ELISA based protein biomarker detection in too slow to be applicable in early patient bedside treatment. Electrical biosensors on the other hand may overcome this limitation with their improved sensitivity, specificity and rapid detection. In this application we demonstrate the development of nanomembrane based electrical protein “nano monitor”. This technique overcomes the limitations current “state-of- the- art” methods such as:• Specificity in detection of multiple markers that characterizes the disease pathogenesis from a single marker to multiple markers.• Speed of detection from a turnaround time of 12/24 hours to a few minutes.• Sensitivity of detection from milligram/ml to nanogram/ml.