Abstract
We present here a novel nano-channel confined biomimetic nanozyme/bioenzyme system, which is prepared by co-immobilizing polyoxometalates (vanadomolybdophosphoric heteropoly acid, PMoV2) and glucose oxidase (GOx) in the nano-scale channel of core-shell mesoporous silica (CSMS) microspheres. The biomimetic dual-enzyme nanoreactors (CSMS@PMoV2@GOx) is used for producing long-lasting chemiluminescence (CL) emission. With glucose and luminol in the reaction mixture, hydrogen peroxide (H2O2) is formed in mesoporous channels by GOx catalysis and then is consumed immediately by PMoV2 to induce CL emission. Confined in nano-size channels of CSMS materials, the biomimetic cascade reactions can induce a long-lasting CL emission with a flat period over 12 h. The proposed CSMS@PMoV2@GOx system has excellent reproducibility and storage stability, and has been applied for glucose sensing in human serum samples with satisfactory accuracy. Our study provides a new glow-type CL system based on nanozyme/bioenzyme cascade reactions confined in nano-scale channels of CSMS microspheres, and will also shed a light on potential application of functional polyoxometalates immobilized in mesoporous materials, which would be of great value in various fields including bioassays and heterogeneous catalysis.
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