Abstract
A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared.It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances.These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.
Highlights
Immobilization of biological material is a problem of prime importance for the biosensor creation
Effect of parameters of Beta polymorph A (BEA) and Linde type A (LTA) zeolites on biosensor operation The effect of addition of different types of zeolites with diverse particle sizes and Si/Al ratios on the analytical characteristics of urea biosensors based on ion-selective field-effect transistors was investigated
The biosensors based on BEA showed higher activity and better sensitivity to substrate compared with the biosensors based on LTA
Summary
Immobilization of biological material is a problem of prime importance for the biosensor creation. Successful immobilization directly influences key characteristics of biosensors (activity of biological material, linear working range, sensitivity to substrate, reproducibility and operational stability of signals, duration of storage, etc.). Selection of main parameters of the immobilization process is an essential challenge in the development of biosensors. An improvement of the methods of biomembrane immobilization onto the sensing element is an important task. One of the trends for solving this problem is an application of adjuvant substances (that are carriers of biomaterial, stabilizing agents, mediators, etc.) during immobilization process. The goal of this work was to study the controlled influence of zeolite crystals on the function of potentiometric biosensors
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