Nano- and micro-polystyrene plastics disturb gut microbiota and intestinal immune system in honeybee

Abstract

Micro- (MPs) and nano-plastics (NPs) have become emerging pollutants in the environment. Their wide distribution and capacity as a vector of hazardous materials threaten various organisms. Honeybees have been used as bioindicators for pollutants as their gut microbiota offers advantages for addressing how it alters the host health and exploring the processes of environmental pollutants affecting gut community dynamics. In this study, the effects of plastic particles of different sizes on honeybees' health were investigated. Oral exposure to polystyrene (PS) particles with a diameter of 100 nm significantly decreased the whole-body weight and survival rate of honeybees and induced intestinal dysplasia. As the increase of the feeding time from Day 0 to Day 15, the MPs moved to and accumulated in the rectum, where most bee gut symbionts colonized. Scanning electron microscope observation showed that 100-nm PS particles adhered to the germination pore of pollen, while 1- and 10-μm PS particles were attached by gut bacteria. We found that 100-nm PS treatment decreased the relative abundance of Lactobacillus and Bifidobacterium in the guts. Correspondingly, PS treatment stimulated immune inhibitory genes and depressed genes related to detoxification and energy balance. Furthermore, 100-nm PS treated honeybees became more susceptible to the pathogenic Hafnia alvei, leading to a five-times higher mortality rate. These results indicated the adverse impacts of NPs on honeybees, which extends our knowledge regarding the emerging health risks of plastic debris, especially at the nanoscale.