Abstract
The high incidence of Diabetes Mellitus in low-income regions has promoted the development of low-cost alternatives to replace blood-based procedures. In this work, we present a bienzymatic paper-based sensor suitable for the naked-eye detection of glucose in saliva samples. The sensor was obtained by a stamping procedure and modified with chitosan to improve the colorimetric readout. The bienzymatic reaction of GOx-HRP coupled with 2,4,6-tribromo-3-hydroxy benzoic acid was applied for the detection of glucose within a range from 0 to 180 mgdL−1 in buffer and artificial saliva solutions. The visual readout was perceived by the naked eye and registered with an office scanner to evaluate the analytical performance. The results showed a limit of detection of 0.37 mgdL−1 (S/N = 3) with an R.S.D. of 1.69% and a linear range from 1 to 22.5 mgdL−1 with an R2 of 0.99235. The analysis of human saliva samples was performed without pre-processing, achieving recoveries from 92 to 114%. The naked-eye detection was evaluated under two different light settings, showing average recoveries of 108.58 and 90.65% for standard and low illumination. The proposed device showed potential for easy-to-use, sensitive, low-cost, fast, and device-free detection of salivary glucose suitable for untrained personnel operation and limited facilities.
Highlights
The high incidence of Diabetes Mellitus (DM) in low- and middle-income countries represents a major concern given the a lack of resources in infrastructure and trained personnel of these regions [1,2,3]
Paper-based sensors were obtained by a stamping method over a cellulose Whatman substrate
We presented the development of a bienzymatic paper-based sensor intended for the detection of glucose in saliva
Summary
The high incidence of Diabetes Mellitus (DM) in low- and middle-income countries represents a major concern given the a lack of resources in infrastructure and trained personnel of these regions [1,2,3]. This has motivated a demand for low-cost, reliable, and easy-to-use platforms for monitoring and diagnosis, which should follow the ASSURED criteria (affordable, sensitive, specific, user friendly, rapid and robust, equipment free, and deliverable) in order to provide results even in the most limited facilities [4]. Manual techniques such as blade coating, manual drawing, and stamping allow rapid prototyping with minimal investment
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