Nakamurella intestinalis sp. nov., isolated from the faeces of Pseudorhynchus japonicus.

Abstract

One strain, designated 63MJ-1T, was isolated from fresh faeces of broad-winged katydids collected in Jinan-gun, Jeollabuk-do, the Republic of Korea. The organism stained Gram-positive and was an aerobic, non-flagellated and short-rod-shaped bacterium. The organism grew in the range of 4-35 °C (optimum, 28-30 °C) and pH 6.0-9.0 (optimum, pH 7.0), and in the presence of 5 % NaCl (w/v), but not in media containing 7 % NaCl. According to the 16S rRNA gene sequence analysis, strain 63MJ-1T showed the highest sequence similarities with Nakamurella panacisegetis P4-7T (95.9 %), Nakamurella endophytica 2Q3S-4-2T (95.8 %) and Nakamurella multipartita DSM 44233T (95.7 %). Phylogenetic trees also indicated that strain 63MJ-1T formed one robust cluster with members of the genusNakamurella. The predominant quinone of strain 63MJ-1T was MK-8(H4). Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified aminophospholipid and two unidentified lipids. The major fatty acids were C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. The peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic amino acid. The DNA G+C content was 64.6 mol%. Based on the phylogenetic, physiological and chemotaxonomic data, it was demonstrated that strain 63MJ-1T represents a novel species of the genus Nakamurella, for which the name Nakamurella intestinalis sp. nov. is proposed. The type strain is 63MJ-1T (=KACC 18662T=NBRC 111844T).