Abstract
1. 1. The assay for NADH-ferrihemoglobin reductase (NADH-FR) was optimized for avian blood samples. 2. 2. In this assay the pH optimum for Japanese quail red cell NADH-FR was 5.5, which was close to the enzyme's pI. 3. 3. Enzyme kinetic parameters were determined for quail, chicken and turkey NADH-FR. 4. 4. Preparation of erythrocyte ghost-cells and subsequent fractionation showed that the enzyme was present in the plasma membrane as well as in the nuclear membrane, while Triton X-100 treatment gave a release of enzyme activity from the membrane. 5. 5. In the cytosolar fraction of avian red cells no NADH-FR could be detected.
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