NADH Autofluorescence Phasor Flim for the Metabolic Characterization of T Cell and Leukemia Cell in a Droplet

Abstract

Immune cells are known to shift their metabolism when stimulated by the interaction with binding receptors. The study of metabolic activation can be very important for the development of immune therapies and for understanding the machinery of immune active cells. However, there are challenges for monitoring real-time cell to cell interactions. Here we report the combination of the autofluorescence NADH Phasor FLIM method with the metabolic fingerprint of Jurkat cells (T lymphocyte) co-encapsulated with K562 (leukemia cells) in a water-in-oil droplet microfluidic chamber.