Abstract
NAD malic enzyme activity was found in the 15 000 g precipitate of citrus leaf and fruit tissues. The enzyme activity in juice vesicle tissue did not change during the fruit growing period, but doubled following ripening. Partially purified enzyme was activated by CoA or FDP. Affinity for malate changed depending on enzyme concentration. The dependency was lost by addition of tricarboxylic acids but not dicarboxylic acids.
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