Abstract
1. 1. NAD + and NADP +dependent isocitrate dehydrogenase (ICDH) activities in homogenates of Crassostrea virginica Gmelin adductor muscle have been separated. 2. 2. Investigation of the kinetic properties of oyster NAD +-dependent ICDH reveals that the K m(IC) is 1.58 mM in the absence of ADP and falls to 0.66 mM in the presence of 1.0 mM ADP. The enzyme is inhibited by nucleoside triphosphates. 3. 3. NAD +-dependent ICDH requires a divalent metal ion for activity [ K m(Mg+2) is 0.61 mM] and is inhibited by iodoacetamide. 4. 4. The results indicate that this NAD +-dependent ICDH activity is a regulatory point of the oyster's Krebs Cycle.
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