Abstract
Neurons were acutely dissociated from the rat nucleus basalis, and whole-cell patch clamp recordings were made. Voltage dependent calcium currents ( I Ca) were recorded and fura-2 microfluorimetric recordings of intracellular free Ca 2+ concentration ([Ca 2+] i) were made at the same time. In Na +-containing solution, a depolarization from −60 to +40 mV evoked the maximal increase in [Ca 2+] i, and this decreased to 43% of the maximal with a large depolarization to +120 mV The [Ca 2+] i increase induced by the large depolarization (+20 to +120 mV) was inhibited by perfusion of Na +-free external solution, and was less when the recording pipette contained a peptide (PRLLFYKYVYKRYRAGKQRG, named XIP) known to inhibit Na/Ca exchange. These results suggest that the [Ca 2+] i increase by the large depolarization is mediated by reverse operation of Na/Ca exchange (Ca 2+ inward and Na + outward).
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