N2O emission dynamics along an intertidal elevation gradient in a subtropical estuary: Importance of N2O consumption

Abstract

Studying nitrous oxide (N2O) production and consumption processes along an intertidal elevation gradient can improve the understanding of N2O dynamics among coastal wetlands. A natural-abundance isotope technique was applied to characterize the processes responsible for N2O emission in high, middle and low intertidal zones in the Yangtze Estuary. The results showed that N2O emission rates in high tidal zones (0.84 ± 0.35 nmol g−1 h−1) were significantly higher than those in middle (0.21 ± 0.04 nmol g−1 h−1) and low tidal zones (0.26 ± 0.05 nmol g−1 h−1). Gross N2O production and consumption rates were greater in high and low tidal zones than in middle tidal zones, whereas N2O consumption proportions generally increased from high to low tidal zones. N2O consumption was quite pronounced, implying that N2O emission in estuarine wetlands accounts for only a small fraction of the total production. Higher degrees of N2O consumption were the pivotal driver of less N2O emission in low tidal zones. Bacterial denitrification (>84%) was the dominant pathway, although hydroxylamine (NH2OH) oxidation/fungal denitrification contributed substantially to N2O production in high tidal flats. The contribution to N2O production exhibited a decrease in NH2OH oxidation/fungal denitrification and an increase in bacterial denitrification with decreasing elevation. Changes in N2O dynamics along the elevation gradient were affected by carbon and nitrogen substrate availabilities as well as the redox environments. Overall, our findings highlight the importance of N2O consumption in controlling N2O emission in intertidal wetlands, especially with higher inundation frequencies and durations.