N2-fixation ability of Brazilian soybean cultivars with Sinorhizobium fredii and Sinorhizobium xinjiangensis

Abstract

The main N2-fixing symbiotic associations with soybean (Glycine max (L.) Merrill) plants are realized with bacteria belonging to the species Bradyrhizobium japonicum and B. elkanii. However, in 1982, fast-growing rhizobia were isolated from soybean root nodules collected in The People's Republic of China and these bacteria are today classified as Sinorhizobium fredii and S. xinjiangensis. The fast growing strains formed an effective symbiosis with primitive soybean cultivars such as Peking, but not with most North American cultivars, which are the progenitors of almost all Brazilian cultivars. The main purpose of this study was to evaluate the ability of 80 soybean cultivars from the Brazilian germplasm bank to produce effective nodules when inoculated with S. fredii or S. xinjiangensis strains. Sixty-six percent of the Brazilian genotypes formed effective nodules with both Sinorhizobium species. However, when 20 Fix+ genotypes were inoculated with a mixture of B. elkanii and S. fredii, at a ratio of 1:1, most or all nodules were occupied by B. elkanii. Consequently, there was no relationship between the growth rate in vitro and the ability to compete for nodule occupancy. Fast-growing strains have also been isolated from soybean nodules in Brazil, but the ecological importance of these symbiotic associations is still to be determined.