N vitro micro propagation of Spilanthes acmella (L.) Murr

Abstract

This study was aimed to In vitro propagation of Spilanthes acmella L. Murr. It is a medicinal plant not cultivated in Iraq. Seeds were sterilized and cultured on MS medium. Indole acetic acid IAA, Benzyladenin BA growth regulators’ were used at the initiation stage. The combination between IAA and BA was used in multiplication stage. Indole butyric acid IBA was used for rooting the shoots. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. A node exhibited relatively highest response as compared with apical meristems and leaflets culture. Supplying the culture medium with 1mg/l. BA was effective for lateral shoot induction. The mean number of shoots obtained from nodes were 7.43 with a mean length 0.9 cm. Adding BA at 0.5, 1.0 or 1.5 and IAA at 0.1 mg/l. to the growth medium was effective for multiplication. Mean number of the developed shoots were 12.00, 10, 84, 10.00 respectively. Adding 0.1, 0.5, 1.0 mg/l IBA to the half strength MS medium was very effective in root formation which produced 45.0, 42.5, 40.0 roots respectively with mean length of 3.25, 3.80, 3.80 cm respectively. Results of acclimatization stage showed that addition of 1:1 Patmos and loamy soil gave the highest rate of survival 100% after 4 weeks of acclimatization. This study showed the ability of in vitro propagation of Spilanthes acmella (L.) Murr