N-Terminal Polypeptide of Annexin A2 Decreases Infection of Mycoplasma hyorhinis to Gastric Cancer Cells.

Shiqin Yuan,Chengchao Shou,Like Qu,Gernot Zissel

doi:10.1371/journal.pone.0147776

Shiqin Yuan, Chengchao Shou + Show 2 more

Open Access

https://doi.org/10.1371/journal.pone.0147776

Copy DOI

Journal: PloS one	Publication Date: Jan 26, 2016
Citations: 13	License type: CC BY 4.0

Affiliation: Peking University, Peking University Cancer Hospital

Abstract

Mycoplasma infection in human and its contamination in cell cultures are worldwide problems. The drugs currently available for preventing or treating mycoplasma infection suffer from low sensitivity, strong resistance and high toxicity. Our previous work showed that Mycoplasma hyorhinis (M. hyorhinis) infection was mediated by the interaction between p37 of M. hyorhinis and Annexin A2 (ANXA2) of host cells, however the translational value of this mechanism was unknown. Herein, we synthesized the N-terminal of ANXA2 polypeptide (A2PP) and found that A2PP could decrease the infection of M. hyorhinis to gastric cancer cells and block M. hyorhinis infection-induced cell migration. Furthermore, we found that A2PP could reduce M. hyorhinis contamination of passage cells. Moreover, compared with the commercial antibiotics commonly used in cell culture to prevent M. hyorhinis infection, A2PP demonstrated a more effectiveness but a low toxicity on cell growth. Thus, our study for the first time revealed A2PP’s potential for the treatment and prevention of M. hyorhinis infection.

Highlights

Pathogenic mycoplasmas, including Mycoplasma pneumoniae (M. pneumoniae), mycoplasma hyorhinis (M. hyorhinis), oral mycoplasma (M.orale) and Mycoplasma genitalium (M. genitalium), belong to class mollicutes, which is the smallest microorganism living in nature and can duplicate independently [1,2]
In order to evaluate the role of Annexin A2 (ANXA2)’s N-terminal domain in M. hyorhinis infection, we firstly synthesized ANXA2 polypeptide (A2PP) peptide corresponding to N-terminal of ANXA2 (Fig 1A)
EGFR has been implicated in regulating ANXA2 phosphorylation [17,25,26], while A2PP had no effects on the phosphorylations or protein levels of EGFR and ANXA2 [Fig 2A]

Summary

Introduction

Pathogenic mycoplasmas, including Mycoplasma pneumoniae (M. pneumoniae), mycoplasma hyorhinis (M. hyorhinis), oral mycoplasma (M.orale) and Mycoplasma genitalium (M. genitalium), belong to class mollicutes, which is the smallest microorganism living in nature and can duplicate independently [1,2]. Many studies showed that infection with these mycoplasmas was associated with tumor development [3,4,5,6,7,8]. M. orale causes chromosome abnormality in human diploid cells and induces cell transformation [6]. Infection with M. hyorhinis or M. genitalium could increase the migration and invasiveness of prostate epithelial cells [3]. M. hyorhinis infection has been linked to arthritis, serositis, infertility and cancer of human [9,10,11,12]. Mycoplasma contamination in cell culture is a serious problem and the rate of passage cells infected by mycoplasma is high. Cell culture is widely used in life sciences, such as in the basic research, clinical trial research, development and production of biological products, as well as in the field of biopharmaceutical and vaccine production.

Methods

Results

Conclusion