Abstract
Lactoferrin (Lf) from buffalo colostrum was probed for N-glycan diversity and abundances that were previously unknown. Out of four N-glycosylation sites present, two sites, N233NT and N545DT, exhibited predominantly high-mannose glycans. Site N281KS showed mainly sialylated hybrid types. A higher abundance of differentially sialylated hybrid glycans was present at N476QT. In addition, the genetic variant of Lf was identified. Overall, this study showed that high mannose and hybrid types are the major glycans in Lf from buffalo colostrum.
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