N-Ethyl-N-Nitrosourea (ENU) induced Osteoporosis in Rats by inducing mutation in Collagen Type 1 protein–A Novel Method Development

Abstract

Osteoporosis is a life-threatening condition that primarily affects women. Mice, rats, dogs, and rabbits have all been used as research animals. Bone strength, bone micro architectural characteristics, and osteoporosis risk are all affected by genetic factors. In this sequence, Type I collagen is the primary bone protein encoded by the COLIA1 and COLIA2 genes. The goal of the study is to develop new model for osteoporosis in rats by using N-Ethyl-N- Nitrosourea (ENU) which particularly targets COL1A1. Further, we treated the osteoporotic rats with Collagen type protein, in order to cross check the disease induction. And Rats induced with ENU, exhibited mutation in COL1A1 gene increased in the levels of both serum and urine biochemical parameters like calcium, phosphorus, ALP and creatinine. PCR analysis reported the mutation in COL1A1 gene. The use of animal models of osteoporosis is required to better understand the complex nature of this condition and to create new preventive and treatment approaches.