Abstract

A green and sensitive analytical procedure by dispersive solid-phase nanoextraction (DSPNE) strategy based on mesoporous nitrogen-doped hollow carbon sphere as adsorbent is developed for the simultaneous determination of atorvastatin (AT) and amlodipine (AM) in spiked human plasma. The determination method was based on measuring the synchronous fluorescence intensity of the drugs at a constant wavelength difference (Δλ = 40 nm). The different experimental parameters influencing the extraction efficiency of the two drugs were studied and optimized. The limits of detection were 3.24 and 0.406 ng. ml−1 and quantification limits were 10.8 and 1.36 ng. ml−1 for AT and AM, respectively. Validation assays indicated that the concentration ranges were linear in 11–120 for AT and 2–90 ng ml−1 for AM, with R2 > 0.993 for both drugs. Under optimum conditions, extraction recovery was 94% and 89% with RSD% 3.9 and 4.7 (n = 4) for AT and AM, respectively. The developed method has been found to be simple, economical, and reproducible for the routine analysis of both drugs in biological fluids.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.