N-cadherin is a prospective cell surface marker of mesenchymal stem cells that determine acceptability for electrical stimulation

Abstract

Background & Aim Background Mesenchymal stem cells (MSCs) are among the most promising sources of stem cells for regenerative medicine. However, multiple tissue-derived MSCs show a wide range of characteristics and our previous study reported electrical stimulation (ES) can drive direct reprogramming of multiple tissue-derived MSCs into hyaline chondrogenic cells. Aim Here, we investigate the aggregation propensity of multiple MSCs producing high- or low-content adhesion molecule under electrical stimulation. Methods, Results & Conclusion Methods Multiple tissue-derived MSCs were primarily cultured from 2-year female beagle dog and cells were analyzed through western blotting using adhesive molecules such as N-cadherin and aggrecan. Images of micromass under ES were captured using a phase-contrast microscope at 40 X magnification and mass was analyzed by qPCR. Results Multiple tissue-derived MSCs show a wide range of characteristics and distinct rates of aggregation under electrical stimulation. We found that N-cadherin, a calcium-dependent cellular adhesive protein, is unnecessary for chondrogenesis to proceed under ES. We showed that a relatively high level of N-cadherin in MSCs results in decreased cellular condensation and impaired chondrogenesis during ES, while low level induces it. Conclusion Careful targeting of this distinctive property could serve as the basis for the optimization of electrical stimulation on multiple-choice of MSCs. Mesenchymal stem cells (MSCs) are among the most promising sources of stem cells for regenerative medicine. However, multiple tissue-derived MSCs show a wide range of characteristics and our previous study reported electrical stimulation (ES) can drive direct reprogramming of multiple tissue-derived MSCs into hyaline chondrogenic cells. Here, we investigate the aggregation propensity of multiple MSCs producing high- or low-content adhesion molecule under electrical stimulation. Multiple tissue-derived MSCs were primarily cultured from 2-year female beagle dog and cells were analyzed through western blotting using adhesive molecules such as N-cadherin and aggrecan. Images of micromass under ES were captured using a phase-contrast microscope at 40 X magnification and mass was analyzed by qPCR.