Abstract
Immunoglobulin A nephropathy ( IgAN ) has been recognized as the most prevalent form of glomerulonephritis with various histologic and clinical phenotypes in the world. The Gal-deficient IgA1 with terminally exposed GalNAc residue(s) plays a key role in the pathogenesis of the disease. In this study, a novel colorimetric biosensor based on Helix aspersa agglutinin (HAA) absorbed on polydiacetylene (PCDA) nanovesicles for N -acetylgalactosamine (GalNAC) molecular as core framework of Gal-deficient IgA1 recognition was investigated using the high affinity between lectin HAA and GalNAC. The PCDA nanovesicles were prepared via UV crosslinking. The size, morphology and elastic property of the nanovesicles were tested. The optimal concentration of HAA for the recognition of GalNAC via naked eyes observation was 3 mM and the critical concentration of GalNAC for the sensitive color transition was 2 μg/mL. This novel method has many advantages such as low prices, intuitive, real-time and easy to carry out and great potential in Gal-deficient IgA1 detection.
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