ウイルスの血球凝集反応の本態に関する研究

Abstract

In this communication a report is made on the mechanism of viral hemagglutination. The results are summarized as follows. I. ELECTROSTATIC CONSIDERATION OF THE VIRAI HEMAGGLUTINATION.The viral hemagglutination were distinguished into two processes, first, adherence of hemagglutinin to the surface of erythrocytes and, second, clot formation of those erythrocytes.The mechanism of the first process was different from other viruses, but the mechanism of the second process was in common with other hemagglutination.The second process was confirmed with various ionic formations of reaction medium, and then, it was nothing else but decrease of negative surface potential of erythrocytes. II. MEASUEMENT OF THE HEMAGGLUTINABILITY OF ERYTHROCYTES.The present author has observed that the erythrocytes disclosed nonspecific hemagglutination in hypertonic concentration of NaCl solution or CaCl2 solution. The nonspecific hemagglutination in hypertonic solution was utilized for measurement of the hemagglutinability.Hypertonic solution (16 times of isotonic concentration) of NaCl or CaCl2 solution was diluted serially twofold by mixing with distilled water and in each 1.0ml of this dilution was added 1 drop of 10% suspension of erythrocytes in normal saline and sedimentation was allowed to proceed at 4°C over night and the patterns were read (according to Salk).It was concluded by the method that hemagglutinability of trypsin and tannic acid treated erythrocytes was increased.