Abstract
6-O-α-maltosyl- and 6-O-α-maltotriosyl-cyclodextrins (CDs) were produced from α-, β-, and γ-CDs and maltose and maltotriose with Pseudomonas isoamylase. The reaction rate was greater with maltotriose than with maltose, and with increasing size of CDs. Besides these branched cyclodextrins, branched hexaoses, 61-, 62-, and 63-maltotriosylmaltotriose in the 1:4.5:1 ratio were formed by the condensation of two maltotrioses. Thus, maltotriose was effective as both a side-chain donor and an acceptor as well, but maltose was only effective as a side-chain donor. The specific activities of isoamylase for the formation of maltotriosyl-CDs were greater than those of pullulanase. The branched CDs and the hexaoses were purified by liquid-gel chromatography and HPLC, and their structures were determined by chemical, enzymic and 13C-NMR spectroscopic analyses.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
