Abstract
In order to study possible cytogenetic effects of argon laser irradiation, V79 Chinese hamster fibroblasts were irradiated with a defocused argon laser beam (100-400 J/cm2). Inhibition of cell growth was observed dose dependently. Flow-cytofluorometric analysis indicated the blockage of cell cycle progression at G2+M phase. Chromosomal effects were investigated by sister chromatid exchange and chromosomal aberration, both of which were elevated dose dependently. Mutation was not induced in ouabain locus. These cytological changes were not due to elevation of temperature during irradiation or experimental artifacts such as the possible phototoxic effects of Kanamycin or phenol red in the culture medium or spontaneous emission of UV light of the laser. These results indicate that the argon laser irradiation at high doses probably induces damage at the chromosomal level.
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