Abstract
A centrifugal partition chromatographic method was developed for separation of grayanotoxin (G)-I, G-II and G-III, which are toxic diterpenoids of Leucothoe grayana MAX. The optimum conditions for separating these grayanotoxins from each other utilized the following: solvent composition, CHCl3:MeOH:H2O=45:60:40 (mobile phase, upper layer; stationary phase, lower layer); rotational speed, 700 rpm; flow rate, 1.0ml/min. The constituents in the eluates were checked by TLC and quantitatively determined by spectrophotometry (at 460 nm) after being treated with vanillin in a solution of perchloric acid.
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