新しい２０００ｍｌバッグによる１０単位濃厚血小板保存の検討

Abstract

The shelf life of 2-unit platelet concentrates (PC) stored in a ordinary 300ml polyvinylchloride (PVC) bag is 72 hours after collection. However, PC obtained by apheresis must be transfused within 24 hours due to the falling of pH which is associated with a loss of viability. We have developed a new 2000ml PVC bag (0.30mm in thickness) with improved gas transmission to store 10-unit PC obtained by apheresis.In our study, 12 units of PC from random donors were pooled in a bag. After immediate gentle mixing, they were then divided into two bags, the new 2000ml bag containd 10 units of PC, the other 300ml bag containd 2 units of PC. Both bags were stored at 22°C and rotated on a horizontal agitator for 96 hours. During storage we monitored platelet count, pH, aggregation response, glucose consumption, lactate production, % hypotonic shock response (%HSR), mean platelet volume (MPV), lactate dehydrogenase (LDH) discharge and morphology score.Mean platelet count did not change during the stroage. The pH became significantly higher in the new 2000ml bag from 24 to 72 hours of storage than that of the 300ml bag (p<0.05). In the new 2000ml bag showed slightly lower lactate production and lower glucose consumption as compared to the ordinary 300ml bag. Aggregation response, %HSR, MPV, LDH discharge and morphology score were approximately the same in comparison with the ordinary 300ml bag.The data indicate that as measured by in vitro response, 10-unit PC obtained by apheresis can be maintained for up to 72 hours if stored in the new 2000ml bag.