Abstract
In order to prevent bacterial contamination during the procedure of plateletapheresis, preconnected platelet rich plasma (PRP) packs were prepared which include the apheresis bowel set of a plasma collecting system (PCS) with a 16 G AVF needle and a double-or triple-bag system. The anticoagulant line of the PRP pack and that from the bag containing acid-citrate-dextrose solution formula A were welded by a sterile connection device (SCD model 312, Du Point). No additional care was needed to perform plateletapheresis with the present closed system. The 388 preparations of platelet concentrates (PCs) tested were demonstrated to be sterile. PCs stored in conventional PVC plastic bags for 3 days at room temperature were evaluated. PC5 with a plasma volume of 100ml containing 1-<2×1011 platelets stored in two 0.6 liter bags and PC10 with a plasma volume of 200-280ml containng 2-4×1011 platelets stored in two or three 1-liter PVC bags maintained their pH at the neutral level for 3 days. The percent hypotonic shock response (%HSR) of platelets was also improved during storage of 3 days. These data suggest that the present closed system will be useful for reducing the risk of bacterial contamination in platelet products during cytapheresis.
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