以杆状病毒为基础的表面展示与基因表达系统

Abstract

杆状病毒表达载体系统已被广泛应用于真核和原核生物的各种蛋白生产。通过与杆状病毒囊膜蛋白gp64融合表达或与特异性的锚定部位结合，可以将外源蛋白质展示在杆状病毒表面。研究发现，携带哺乳动物启动子的重组杆状病毒能将外源基因传递入哺乳动物细胞，实现外源蛋白的有效表达，表达蛋白的结构更接近天然蛋白。杆状病毒作为一种新型的基因表面展示和基因传递系统，具有广阔的应用前景，本文对杆状病毒作为外源蛋白表面展示技术和作为基因传递载体在哺乳动物细胞中表达外源基因的研究进展进行了综述。 The baculovirus expression vector system has been used extensively to produce numerous proteins originating from both prokaryotic and eukaryotic sources. Through fusing to the entire membrane protein gp64 of baculovirus or combining with special membrane anchor domain, exotic proteins could be expressed on the surface of baculovirus. It was found that baculovirus harboring mammalian expression cassettes could efficiently deliver foreign genes into mammalian cells and successfully express foreign genes. The baculovirus as surface display and gene delivery vector in mammalian cells have extensive prospects. In this article, the progress of baculovirus surface display technology and gene delivery system in mammalian cells was reviewed.