Abstract
Limonoids in seeds of Sudachi (Citrus sudachi Hort. ex Shirai) were analyzed by high pressure liquid chromatography with a U. V. spectrophotometer. Sudachi seeds contained all of the major limonoids commonly found in other citrus. Limonin was predominant (2.53mg per gram of fresh weight, 50.3% of the total limonoids), followed by ichangensin, deacetylnomilin, nomilin, ichangin and obacunone. The seeds also contained common acidic limonoids such as nomilinic acid, deacetylnomilinic acid and isolimonic acid.The presence of ichangensin supports the previous suggestion that Sudachi is related to Yuzu (Citrus junos Sieb. ex Tanaka) and Ichang Chie (Citrus ichangensis Swingle). Ichangensin in Sudachi is most likely inherited from Ichang Chie which has been shown to possess this compound as the predominant limonoid.
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