两种浒苔无机碳利用对温度响应的机制

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 两种浒苔无机碳利用对温度响应的机制 DOI: 10.5846/stxb201304200756 作者: 作者单位: 淮海工学院海洋学院,淮海工学院海洋学院,淮海工学院海洋学院,淮海工学院海洋学院 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金资助项目（41106093）；江苏省自然科学基金资助项目（BK2011400）；国家海洋局海洋公益性行业科研专项资助项目（201205010）；江苏高校优势学科建设工程资助项目 The mechnism of the characters of inorganic carbon acquisition to temperature in two Ulva species Author: Affiliation: School of Marine Science ＆ Technology,Huaihai Institute of Technology,Lianyungang,Jiangsu,China,School of Marine Science ＆ Technology,Huaihai Institute of Technology,Lianyungang,Jiangsu,China,School of Marine Science ＆ Technology,Huaihai Institute of Technology,Lianyungang,Jiangsu,China,School of Marine Science ＆ Technology,Huaihai Institute of Technology,Lianyungang,Jiangsu,China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为了探讨温度对大型海藻无机碳利用机制的影响，选择了潮间带常见的绿藻缘管浒苔 （Ulva linza）和浒苔（Ulva prolifera）为实验材料，研究了碳酸酐酶抑制剂乙酰唑磺胺（AZ）和己氧苯并噻唑磺胺（EZ）在不同的温度下对藻体光合作用的影响。实验设置6个温度梯度（5、10、15、20、25和30℃）。结果表明，缘管浒苔和浒苔都有很强的无机碳利用能力，而温度对此有显著的影响。但他们之间存在明显的种间差异。缘管浒苔对温度的依赖性要强于浒苔，其对温度的适应范围要窄于浒苔，尤其是在高温下（30℃），缘管浒苔的最大光合作用能力与最适温度相比下降了56%，而浒苔仅为20%，这表明在高温的情况下，浒苔比缘管浒苔具有更强的生存适应能力，这是浒苔能够在绿潮藻占有绝对优势的原因之一。对缘管浒苔来说，在低温（5℃）和高温（30℃）时，无机碳的转运主要是通过胞外碳酸酐酶的催化作用，而在15℃时，加入胞外碳酸酐酶的抑制剂对无机碳的转运没有明显的影响，这表明在此温度下其他无机碳转运形式可以有效补偿胞外碳酸酐酶的作用。在其他的温度下，胞外碳酸酐酶和其他无机碳转运方式各占一定的比例。而对于浒苔来说，在低温5℃时，其他无机碳转运形式占主要作用，而从10℃开始，胞外碳酸酐酶作用比例显著增加，并且保持在相同的水平上。 Abstract:The intertidal green macroalgae Ulva linza and Ulva prolifera were selected to study the effects of carbonic anhydrase inhibitors AZ and EZ on the photosynthesis of the thalli under different temperatures. Six temperature levels were set (5, 10, 15, 20, 25 and 30℃) in the experiment. Our results showed that the values of pH compensation point of both Ulva linza and Ulva prolifera was as high as 10. This suggested that the both the two Ulva species possessed rather high ability of photosynthetic acquisition of inorganic carbon. The ability of inorganic carbon acquisition in both the two species were significantly affected by temperature, and this temperature responses were species-specific. Compared with Ulva prolifera, Ulva linza was more sensitive to temperature, suggesting that the optimum range of temperature for photosynthesis was narrower in Ulva linza than Ulva prolifera. At high temperature (30℃), the net photosynthetic rate of Ulva linza was decreased by 56%, while only by 20% in Ulva prolifera, compared with the rate at optimum temperature. These results suggested that Ulva prolifera possessed stronger ability to endure high temperature than Ulva linza did. This might be one of the most possilble physiological reasons that the dominant species in green tide bloom is Ulva prolifera. For Ulva linza, the transport of inorganic carbon was predominantly carried out by catalysis of extracellular carbonic anhydrase at temperatures of 5℃ and 30℃. However, no significant effect was found in the transport of inorganic carbon at temperature of 15℃ when the extracellular carbonic anhydrase inhibitor AZ was added. This implied that, at 15℃, another way of transport of inorganic carbon might eliminate the effect of the inhibition of extracellular carbonic anhydrase. Over the temperature rang of 20-25℃, extracellular carbonic anhydrase-mediated inorganic carbon transport and the other patterns of inorganic carbon transport operated as a fixed proportion, respectively. For Ulva prolifera, the primary pattern of the transport of inorganic carbon was not due to the catalysis of extracellular carbonic anhydrase at temperature of 5℃, and another pattern of inorganic carbon transport might operate at this temperature. However, as temperature rose to 10℃, the action of catalysis of extracellular carbonic anhydrase increased significantly, and this action maintained the same levels with further rising of temperature. 参考文献 相似文献 引证文献