Abstract
Microsporogenesis, male germ unit formation during pollen germination and pollen tube elongation in pistils were observed on the tetraploid (4X) Hyuganatsu (Citrus tamurana Hort. ex Tanaka).1. In anthers of the 4X Hyuganatsu nearly 25% of the pollen mother cell (PMC) degenerated, while in the remaining PMC, 1 to 9 sporads resulted from abnormal meiosis. Thus, the size and shape of the pollens were not uniform but malformed. Abnormally large and small pollens were observed. Pollen viability of the 4X Hyuganatsu as ascertained by FDA staining procedure was lower than that of the 2X Hyuganatsu.2. In the pollen tubes of the 4X Hyuganatsu, the normal male germ unit consisting of 2 sperm nuclei and 1 vegetative nucleus was not formed regularly; 46.4% of the pollen tubes cultured in vitro did not contain any nucleus even after 22 hr at 30°C.3. When stigmas of the 2X Hyuganatsu were pollinated with the 4X Hyuganatsu pollens, the pollen tubes grew normally through the style and penetrated the ovaries 9 days after pollination.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.