ジステンパーの中和抗体に関する実験

Abstract

Distemper neutralizing antibodies in sera of various immunized dogs and guinea pigs were investigated by means of neutralization tests using the chorioallantoic membrane of the chick embryo and an egg-adapted strain of distemper virus as indicative virus. The results were as follows.1. The presence of neutralizing antibodies was hardly indicated in sera collected from three-to four-month-old puppies, but was indicated with remarkably high titers.2. Very variable titers of neutralizing antibodies were shown in sera collected from 18 dogs clinically diagnosed as distemper.3. In the case of guinea pigs inoculated intraperitoneally and of dogs inoculated subcutaneously with the egg-adapted distemper virus or a formolized tissue vaccine, neutralizing antibodies in sera of the guinea pigs indicated the highest level on the 21st day after the inoculation and those in sera of the dogs the highest level between 14 and 42 days. In addition, the production of neutralizing antibodies in sera collected from guinea pigs was in proportion to the inoculated dose of virus. The neutralizing antibody levels were exceedingly low in both dogs and guineapigs receiving formolized tissue vaccines.4. Neutralizing antibodies were found in abundance in commercial immune sera or gammaglobulin products.