Abstract
This commentary describes the advantages of laser spray, which was developed by Hiraoka et al., in the analysis of binding affinities of biomolecular complexes, such as DNA-protein and DNA-drug complexes. Since laser spray enables dissociation of the complexes without fragmentation of labile compounds such as DNA, a good correlation was found between the binding affinities and applied laser power for dissociation. We found that laser spray is promising for rapid characterization of binding affinities of complexes with small differences in structure.
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