Abstract
Studies on shape change associated with movement of fibroblasts are mainly based on two-dimensional observation and analysis. Since the movement of fibroblasts is slow and complicated, few analytical studies have focused on three-dimensional cell movement. In this study, we observed three-dimensional shape change associated with movement of fibroblasts. Swiss 3T3 mouse fibroblasts were transiently transfected with TagGFP-lifeact, and actin-labeled cells were placed on a glass substrate coated with 30 μg/mL fibronectin with PBS for 30 minutes. Time-lapse live cell images were obtained by confocal laser scanning microscopy, and cell shape was measured from three-dimensional actin- labeled images. As a result, changes in z-coordinate of volume center (VC) were lowly related to movement of fibroblasts. Moreover, z-coordinate of base area center (BC) steps forward from z-coordinate of VC. Further study would be needed to clarify the reason why the movement of the VC and BC linked based on the molecular dynamics of the intercellular structure.
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