Abstract
We have constructed a fluorescence lifetime imaging (FLIM) system to study the photo-induced dynamics of living cells. The output from a femtosecond mode-locked Ti: sapphire laser is frequency-doubled and focused onto the sample with an objective lens of a confocal microscope. The fluorescence decay of the sample is measured at each pixel of a scanning image. Each fluorescence lifetime is evaluated quickly using time-gating electronics, and the fluorescence lifetime image can be obtained within several minutes. We have combined the FLIM system with a picosecond time-correlated single-photon counting system in which a microchannel-plate photomultiplier is used for detection, so that the value of the fluorescence lifetime at a position can be analyzed quantitatively. A high contrast light-scattering image of a polymer film is also found to be obtained with the FLIM system.
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